To reduce the chemical load on the soil, plants and fruits, it is important to use alternative methods of soil and plant cultivation, which include irradiation with non-ionizing radiation. To establish the range of technological parameters and operating modes of the developed machines for tillage with non-ionizing radiation, the authors developed a technique and an experimental installation capable of providing infrared and ultraviolet radiation. To study the soil intended for use as protected greenhouse ground, standard methods for determining the physical, microbiological, and qualitative properties of the soil were applied. In the course of research, five modes of soil cultivation were implemented: infrared radiation for three hours with and without the mechanical mixing of the soil; ultraviolet radiation for one hour; exposure for one hour to one infrared and one ultraviolet emitters with the mechanical mixing of the soil; processing for one hour with three infrared emitters and one ultraviolet with the mechanical mixing of the soil. Microbiological studies of the soil treated at the experimental unit were carried out within one hour after the selection of the material according to the presented method. It has been experimentally established that, depending on the modes of soil cultivation, the number of microorganisms decreases by 14…98%. The combination of infrared and ultraviolet radiation with a simultaneous threefold increase in the thermal power of radiation for one hour helps to reduce the number of microorganisms up to 98%. The decrease in the content of microorganisms in the soil due to non-ionizing radiation is the first step to establish the design and technological parameters and operating modes of the developed machines used for tillage in protected greenhouse conditions.